Ape da miele (apis mellifera ligustica) Honeybees (Apis mellifera ligustica) are essential pollinators in both ecosystems and agriculture and its practices. However, their populations are declining due to various factors, including exposure to pesticides. Despite their importance, their reproductive castes, particularly drones, remain poorly studied in terms of pesticide effects. Particular attention was paid to fungicides widely used in agriculture and which, according to popular belief, are not harmful to the protection of bees and pollinators in general. Fungicida a base di azossistrobina e pubblicizzato come "selettivo e tutelante gli impollinatori" To study the effects of azoxystrobin and picoxystrobin on honeybee drones, the drones were exposed to different concentrations of azoxystrobin and picoxystrobin for 14 days; drone survival and body weight, as well as reproductive organs, sperm concentration, and nutrient content, were assessed. This study demonstrated that exposure to both fungicides caused a significant reduction in drone survival, with survival rates progressively decreasing as the duration of exposure increased. Compared to the control group, the body weights of drones in all treatment groups were significantly lower at days 7 and 14 of life. Nutrient analysis revealed that low concentrations of azoxystrobin and picoxystrobin increased protein levels, while free fatty acid content decreased significantly in all treatment groups. No significant changes in total carbohydrate content were observed. Fungicida ad ampio spettro a base di picossistrobina utilizzato per il trattamento del frumento (tenero e duro), orzo, segale, ecc. Morphological examination of the reproductive organs showed that the lengths of the mucous glands and seminal vesicles in the drones were significantly shorter in the treatment groups than in the control group. Furthermore, exposure to azoxystrobin and picoxystrobin caused a significant decrease in sperm concentration in the drones. These results indicate that azoxystrobin and picoxystrobin have a negative effect on the health and reproductive capacity of honeybee drones . This study provides new insights into the adverse effects of azoxystrobin and picoxystrobin on honeybee drones. The significant impact on survival, body weight, nutrient content, reproductive organs, and sperm concentration emphasizes the need to reassess the risks posed by these fungicides to pollinators. Given the crucial role of drones in preserving the genetic diversity and resilience of honeybee colonies, these findings have significant implications for beekeeping and agricultural practices. Future research should focus on clarifying the mechanisms underlying these effects and exploring potential strategies to mitigate the impact of fungicides on pollinator health. Furthermore, long-term studies are needed to assess the cumulative impact of fungicide exposure on bee populations and colony dynamics. Published: July 24, 2025 Full text: https://www.mdpi.com/2077-0472/15/15/1590 Authors: Wenlong Tong 1,†, Lizhu Wang 1,†, Bingfang Tao 1, Huanjing Yao 1, Huiping Liu 2,3, Shaokang Huang 1,4 Jianghong Li 1,4, Xiaolan Xu 1,4 and Xinle Duan 1,2,4,* 1 College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China 4 Fujian Honey Bee Biology Observation Station, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China